Wednesday, July 17, 2013
Rat Cardiac Myocytes
This video shows the changes in optical thickness (OT) of spontaneously beating rat cardiac myocytes as imaged with the BioCam Quantitative Phase Microscope. The myocytes were measured in a Bioptechs FCS3 perfusion chamber with an aluminized base plate and a 100μm gasket. Cells were kept at 37°C with an HBSS (Hank’s balanced salt solution) fluid bath. The video on the left shows a 2D representation of the OT data, while the video at right shows the data in 3D. The maps were acquired at 15fps.
