Live Cell Measurements: Oligodendrocytes, Myocytes and Myoblasts

4D Technology recently measured various live cells while the BioCam Quantitative Phase Microscope was on site at a pharmaceutical research organization. Here are several of the videos—we are in the process of quantifying various aspects of these experiments.

Oligodendrocytes measured over 30 minutes with data taken once per minute. Stretching, contraction, and bending of the various processes (branches) can be observed over this time frame.

Myocytes measured over a 211x211 um field of view. The beginning of the cell fusion process is observed as well as flapping of the plasma membranes as the cells stretch and reorient themselves in preparation for fusion. This time series was taken at 10 second intervals.

Myoblasts with multiple bursts measured at 10 frames/second over a period of an hour showing vesicles moving through the cell.

4D Presentation at BIOS San Francisco, Feb. 6

Dr. Goldie Goldstein will be presenting “Quantitative Phase Microscopy: How to Make Phase Data Meaningful” at BIOS in San Francisco, Thursday, Feb 6, 10:50-11:10am in Room 304 (Esplanade). Please join Drs. Goldstein and Creath for this informative presentation. If you’d like to arrange a time to meet them separately you can e-mail kathy.creath@4dtechnology.com.

4D Presentation at Quantitative BioImaging Conference

In January Dr. Kathy Creath and Dr. Goldie Goldstein presented “Real-Time Quantitative Optical Volume Measurement of Dynamic Cellular Motion” at the Quantitative BioImaging Conference in Albuquerque. The conference was an excellent venue to explore how quantitative phase microscopy can be used in conjunction with other techniques to extract more information from studies.